English: “Mini-brains” representative of different brain region identities were generated in Yan Liu’s laboratory. a Immunofluorescence image of cortical organoids grown from pluripotent stem cells (PSCs) with multiple rosette-like structures. Shown are immunohistochemical staining images for the adherens junction marker PKCλ (green), the progenitor marker SOX2 (red) and the proliferative marker KI67 (green) after 30 days of differentiation. b Immunofluorescence of the midbrain dopamine (DA) neuron marker TH (green), the floor plate progenitor marker FOXA2 (red) and PKCλ (green) at 50 days in midbrain organoids. c Image of striatal organoids stained for the medium spiny neuron (MSN) marker DARPP32 (green) and the mature neuron marker NEUN (green). d Staining for KI67 (green) and SOX2 (red) in cerebellar organoids at 30 days. Scale bar, 100 μm